Chromosomes paternels et maternels

Fréquence et facteurs de risque maternels de la mort fœtale in utero à Kamina, République Démocratique du Congo

INTRODUCTION Fetal intrauterine death constitutes a frequent problem in obstetric practice. The objectives of this study were to determine the frequency and to identify risk factors of fetal death at Reference General Hospital of Kamina. METHODS The study was done in two times. First, a cross-sectional descriptive study of 379 deliveries which allowed to determine the frequency of fetal death...

Point mutation of bacterial artificial chromosomes by ET recombination.

Bacterial artificial chromosomes (BACs) offer many advantages for functional studies of large eukaryotic genes. To utilize the potential applications of BACs optimally, new approaches that allow rapid and precise engineering of these large molecules are required. Here, we describe a simple and flexible two-step approach based on ET recombination, which permits point mutations to be introduced i...

Rapid modification of bacterial artificial chromosomes by ET-recombination.

We present a method to modify bacterial artificial chromosomes (BACs) resident in their host strain. The method is based on homologous recombination by ET-cloning. We have successfully modified BACs at two distinct loci by recombination with a PCR product containing homology arms of 50 nt. The procedure we describe here is rapid, was found to work with high efficiency and should be applicable t...

Leptospirose Ovine et Caprine

Shuffling Chromosomes

The gene order of chromosomes can be rearranged by chromosomal inversions that reverse the order of segments. Motivated by a comparative study of two Drosophila species, we investigate the number of reversals that are needed to scramble the gene order when all reversals are equally likely and when the segments reversed are never more than L genes. In studying this question we prove some new res...